{"{0} ambiguous bases":"{0} 个模糊碱基","{0} amino acids":"{0} 个氨基酸","{0} bases":"{0} 个碱基","{0} bp":"{0} bp","{0} live of {1} counted":"已计数的{1}个中有{0}个活细胞","2 × (A + T) + 4 × (G + C), for short primers":"2 × (A + T) + 4 × (G + C)，适用于较短的引物","Chance":"概率","Complement":"互补序列","Copy":"复制","Dead (stained) cells in each square, optional":"每个方格中的死细胞（染色细胞），可选","Different genotypes":"不同基因型","Dilution factor (2 for a 1 : 1 mix with trypan blue)":"稀释倍数（与台盼蓝按1 : 1混合时为2）","DNA or RNA sequence (FASTA headers, numbers, and spaces are ignored)":"DNA或RNA序列（忽略FASTA标题、数字和空格）","DNA sequence tools":"DNA序列工具","Each parent passes on one allele of each gene, chosen at random and independently for different genes. The square lists every combination of the parents' gametes; with complete dominance, a capital letter hides a small one, so Aa looks like AA. Two heterozygous parents give the classic 3 : 1 ratio for one gene and 9 : 3 : 3 : 1 for two. Linked genes, incomplete dominance, and sex-linked traits follow other ratios.":"每位亲本会将每个基因的一个等位基因传给后代；对于不同基因，传递的等位基因是随机且相互独立选出的。方格列出亲本配子的所有组合；在完全显性情况下，大写字母会掩盖小写字母，因此Aa看起来与AA相同。两个杂合亲本在一个基因时产生经典的3 : 1比例，在两个基因时产生9 : 3 : 3 : 1比例。连锁基因、不完全显性和伴性状会遵循其他比例。","Enter a primer of at least 6 bases using only A, C, G, and T.":"输入至少含6个碱基的引物，只能使用A、C、G和T。","Enter the live count for each square you counted and a dilution factor of 1 or more.":"输入每个已计数方格中的活细胞数，并输入不小于1的稀释倍数。","frame {0}":"阅读框{0}","GC content":"GC含量","Genotype":"基因型","Give a dead count for each square, or leave the dead counts empty.":"输入每个方格中的死细胞数，或将死细胞数留空。","Hemocytometer calculator":"血球计数板计算器","Length":"长度","Live cells counted in each large square":"每个大方格中计数的活细胞数","Live cells in the suspension":"悬液中的活细胞","Live cells per mL":"每mL活细胞数","Longest open reading frame":"最长开放阅读框","Longest ORF protein":"最长ORF蛋白","may form hairpins or primer dimers":"可能形成发夹结构或引物二聚体","Na⁺ concentration (mM)":"Na⁺浓度（mM）","None":"无","None of 4 or more":"4个或更多中无一个","On an improved Neubauer chamber each large corner square holds 0.1 µL (1 mm × 1 mm × 0.1 mm), so cells per mL = average count per square × dilution factor × 10,000. Count the four corner squares, including cells on two of the edges but not the other two, and aim for 20–200 cells per square; dilute more if there are more. Trypan blue enters dead cells and stains them blue; mixing equal volumes doubles the dilution.":"在改良Neubauer计数板中，每个角落大方格的体积为0.1 µL（1 mm × 1 mm × 0.1 mm），因此每mL细胞数 = 每个方格的平均计数 × 稀释倍数 × 10,000。计数四个角落方格，将位于其中两条边上的细胞计入，但不计入另外两条边上的细胞；每个方格以计数20–200个细胞为宜，数量更多时应进一步稀释。台盼蓝会进入死细胞并将其染成蓝色；等体积混合会使稀释倍数加倍。","Parent 1 genotype":"亲本1基因型","Parent 2 genotype":"亲本2基因型","Paste a sequence in plain text or FASTA format; U is read as T, and anything that is not a base letter is removed. Translation uses the standard genetic code, with * for stop codons and X for codons containing ambiguous bases. Frames +1 to +3 read the strand as given from the first, second, and third base; −1 to −3 read the reverse complement. The longest ORF runs from an ATG to the next stop, or to the end of the sequence.":"粘贴纯文本或FASTA格式的序列；U按T读取，所有不是碱基字母的内容都会被删除。翻译使用标准遗传密码，终止密码子用*表示，含有模糊碱基的密码子用X表示。阅读框+1至+3分别从给定链的第一个、第二个和第三个碱基开始读取；−1至−3读取反向互补链。最长ORF从ATG开始，延续至下一个终止密码子或序列末尾。","Paste a sequence of at least three bases.":"粘贴至少含三个碱基的序列。","Phenotype (dominant written as A_)":"表型（显性性状写作A_）","Phenotype ratio":"表型比例","Primer concentration (nM)":"引物浓度（nM）","Primer sequence (5′ to 3′)":"引物序列（5′到3′）","Primer Tm calculator":"引物Tm计算器","Punnett square calculator":"潘尼特方格计算器","Reverse complement":"反向互补序列","SantaLucia 1998, with salt correction":"SantaLucia 1998，含盐校正","Self-complementary stretch":"自互补片段","Suspension volume (mL, optional)":"悬液体积（mL，可选）","The nearest-neighbour method adds up the stability of each overlapping pair of bases, using SantaLucia's 1998 unified values with his salt correction, and gives the temperature at which half the primer is bound; with C/4 for the strand concentration, as Primer3 uses, it agrees with Biopython's Tm_NN to 0.01 °C. The Wallace rule and the GC formula are quicker approximations. Good PCR primers are usually 18–25 bases with 40–60% GC, and a primer pair should be within about 5 °C of each other; a typical annealing temperature is 3–5 °C below the lower Tm. Mg²⁺ and dNTPs, which this does not model, also raise Tm.":"最近邻方法使用SantaLucia 1998年的统一数值及其盐校正，将每一对重叠碱基的稳定性相加，并给出一半引物结合时的温度；按照Primer3的做法，以C/4作为链浓度时，其结果与Biopython的Tm_NN相差不超过0.01 °C。Wallace规则和GC公式是更快的近似方法。优质PCR引物通常含有18–25个碱基，GC含量为40–60%；一对引物的Tm相差应在约5 °C以内；典型的退火温度比较低的Tm低3–5 °C。此工具未建模的Mg²⁺和dNTP也会升高Tm。","Tm, GC formula":"Tm，GC公式","Tm, nearest neighbour":"Tm，最近邻法","Tm, Wallace rule":"Tm，Wallace规则","Total cells per mL":"每mL总细胞数","Translation in six frames":"六个阅读框翻译","Viability":"存活率","Write each genotype as pairs of letters, one pair per gene, the same genes for both parents: Aa, AaBb, or AaBbCc. Capital letters are dominant.":"将每个基因型写成一对字母，每个基因一对，且两位亲本的基因相同：Aa、AaBb或AaBbCc。大写字母表示显性。"}