{"{0} ambiguous bases":"{0} 個模糊鹼基","{0} amino acids":"{0} 個胺基酸","{0} bases":"{0} 個鹼基","{0} bp":"{0} bp","{0} live of {1} counted":"已計數的{1}個中有{0}個存活","2 × (A + T) + 4 × (G + C), for short primers":"2 × (A + T) + 4 × (G + C)，適用於短引子","Chance":"機率","Complement":"互補序列","Copy":"複製","Dead (stained) cells in each square, optional":"每個方格中的死亡（染色）細胞，可選填","Different genotypes":"不同基因型","Dilution factor (2 for a 1 : 1 mix with trypan blue)":"稀釋倍數（與台盼藍以1 : 1混合時為2）","DNA or RNA sequence (FASTA headers, numbers, and spaces are ignored)":"DNA或RNA序列（會忽略FASTA標頭、數字和空格）","DNA sequence tools":"DNA序列工具","Each parent passes on one allele of each gene, chosen at random and independently for different genes. The square lists every combination of the parents' gametes; with complete dominance, a capital letter hides a small one, so Aa looks like AA. Two heterozygous parents give the classic 3 : 1 ratio for one gene and 9 : 3 : 3 : 1 for two. Linked genes, incomplete dominance, and sex-linked traits follow other ratios.":"每位親代會將每個基因的一個等位基因傳給子代；不同基因的選擇彼此獨立且為隨機。方格會列出親代配子的所有組合；在完全顯性下，大寫字母會掩蓋小寫字母，因此Aa看起來像AA。兩位雜合親代在一個基因時會產生經典的3 : 1比例，在兩個基因時則為9 : 3 : 3 : 1。連鎖基因、不完全顯性和性聯遺傳性狀會遵循其他比例。","Enter a primer of at least 6 bases using only A, C, G, and T.":"請輸入至少6個鹼基的引子，且只能使用A、C、G和T。","Enter the live count for each square you counted and a dilution factor of 1 or more.":"請輸入每個已計數方格的存活細胞數，以及大於或等於1的稀釋倍數。","frame {0}":"讀框{0}","GC content":"GC含量","Genotype":"基因型","Give a dead count for each square, or leave the dead counts empty.":"請輸入每個方格的死亡細胞數，或將死亡細胞數留空。","Hemocytometer calculator":"血球計數板計算機","Length":"長度","Live cells counted in each large square":"每個大方格中計數的存活細胞","Live cells in the suspension":"懸浮液中的存活細胞","Live cells per mL":"每mL存活細胞數","Longest open reading frame":"最長開放閱讀框","Longest ORF protein":"最長ORF蛋白質","may form hairpins or primer dimers":"可能形成髮夾結構或引子二聚體","Na⁺ concentration (mM)":"Na⁺濃度（mM）","None":"無","None of 4 or more":"4個以上皆無","On an improved Neubauer chamber each large corner square holds 0.1 µL (1 mm × 1 mm × 0.1 mm), so cells per mL = average count per square × dilution factor × 10,000. Count the four corner squares, including cells on two of the edges but not the other two, and aim for 20–200 cells per square; dilute more if there are more. Trypan blue enters dead cells and stains them blue; mixing equal volumes doubles the dilution.":"在改良式Neubauer計數板中，每個角落大方格的容積為0.1 µL（1 mm × 1 mm × 0.1 mm），因此每mL細胞數 = 每方格平均計數 × 稀釋倍數 × 10,000。請計數四個角落方格，包含其中兩邊上的細胞，但不包含另外兩邊上的細胞；每個方格以20–200個細胞為目標，若數量更多，請進一步稀釋。台盼藍會進入死亡細胞並將其染成藍色；等體積混合會使稀釋倍數加倍。","Parent 1 genotype":"親代1基因型","Parent 2 genotype":"親代2基因型","Paste a sequence in plain text or FASTA format; U is read as T, and anything that is not a base letter is removed. Translation uses the standard genetic code, with * for stop codons and X for codons containing ambiguous bases. Frames +1 to +3 read the strand as given from the first, second, and third base; −1 to −3 read the reverse complement. The longest ORF runs from an ATG to the next stop, or to the end of the sequence.":"請貼上純文字或FASTA格式的序列；U會視為T，任何不是鹼基字母的內容都會移除。翻譯使用標準遺傳密碼，以*表示終止密碼子，以X表示包含模糊鹼基的密碼子。+1至+3讀框分別從第一、第二和第三個鹼基開始讀取原序列；−1至−3讀框則讀取反向互補序列。最長ORF會從ATG延伸至下一個終止密碼子，或延伸至序列末端。","Paste a sequence of at least three bases.":"請貼上至少包含三個鹼基的序列。","Phenotype (dominant written as A_)":"表現型（顯性寫作A_）","Phenotype ratio":"表現型比例","Primer concentration (nM)":"引子濃度（nM）","Primer sequence (5′ to 3′)":"引子序列（5′至3′）","Primer Tm calculator":"引子Tm計算機","Punnett square calculator":"Punnett方格計算機","Reverse complement":"反向互補序列","SantaLucia 1998, with salt correction":"SantaLucia 1998，含鹽校正","Self-complementary stretch":"自我互補片段","Suspension volume (mL, optional)":"懸浮液體積（mL，可選填）","The nearest-neighbour method adds up the stability of each overlapping pair of bases, using SantaLucia's 1998 unified values with his salt correction, and gives the temperature at which half the primer is bound; with C/4 for the strand concentration, as Primer3 uses, it agrees with Biopython's Tm_NN to 0.01 °C. The Wallace rule and the GC formula are quicker approximations. Good PCR primers are usually 18–25 bases with 40–60% GC, and a primer pair should be within about 5 °C of each other; a typical annealing temperature is 3–5 °C below the lower Tm. Mg²⁺ and dNTPs, which this does not model, also raise Tm.":"最近鄰法會使用SantaLucia 1998年的統一數值及其鹽校正，累加每一對重疊鹼基的穩定性，並計算出一半引子與其結合時的溫度；按照Primer3的做法，以C/4作為鏈濃度時，結果與Biopython的Tm_NN相差不超過0.01 °C。Wallace規則和GC公式是較快速的近似方法。良好的PCR引子通常有18–25個鹼基，GC含量為40–60%；一對引子的Tm彼此差距應在約5 °C以內，典型的退火溫度比較低的Tm低3–5 °C。本工具未建模的Mg²⁺和dNTP也會提高Tm。","Tm, GC formula":"Tm，GC公式","Tm, nearest neighbour":"Tm，最近鄰法","Tm, Wallace rule":"Tm，Wallace規則","Total cells per mL":"每mL總細胞數","Translation in six frames":"六個讀框的翻譯","Viability":"存活率","Write each genotype as pairs of letters, one pair per gene, the same genes for both parents: Aa, AaBb, or AaBbCc. Capital letters are dominant.":"請將每個基因型寫成字母對，每個基因一對，且兩位親代使用相同的基因：Aa、AaBb或AaBbCc。大寫字母代表顯性。"}